Biotin and chromium histidinate improve glucose metabolism and proteins expression levels of IRS-1, PPAR-γ, and NF-κB in exercise-trained rats

Table 1 Primer sequences of genes used for real-time polymerase chain reaction

Gene	Primer	Sequence
IRS-1	Forward	5′-GCGCAGGCACCATCTCAACAACC-3′
IRS-1	Reverse	5′-GCACGCACCCGGAAGGAACC-3′
PPAR-γ	Forward	5′-TCAAACCCTTTACCACGGTT-3′
PPAR-γ	Reverse	5′-CAGGCTCTACTTTGATCGCA-3′
NF-κB	Forward	5′-TGAGGCTGTTTGGTT TGAGA −3′
NF-κB	Reverse	5′-TTATGGCTGAGGTCTGGTCTG-3′
GAPDH	Forward	5′-TGATGACATCAAGAAGGTGGTGAAG-3′
GAPDH	Reverse	5′TCCTTGGAGGCCATGTGGGCCAT-3′

IRS-1 insulin receptor substrate-1, PPAR-γ peroxisome proliferator-activated receptor gamma,
NF-κB nuclear factor kappa B

ISSN: 1550-2783